EvaGreen® 20X in Water, 1 mL
EvaGreen® 20X in Water is a fluorescent nucleic acid dye designed for real-time PCR (qPCR), DNA melt curve analysis, high-resolution melting (HRM®), loop-mediated isothermal amplification (LAMP), and digital PCR. The dye exhibits low fluorescence in its free form and produces a stronger fluorescent signal when bound to double-stranded DNA (dsDNA), supporting fluorescence-based DNA detection and analysis.
Product Description
EvaGreen® Dye, 20X in Water, is a DNA-binding fluorescent reagent developed for molecular biology applications that require fluorescence-based detection of double-stranded DNA. Its fluorescence increases upon binding to dsDNA, making it useful for monitoring DNA amplification during real-time PCR and for analyzing amplification products after a reaction.
The 20X aqueous formulation provides a concentrated dye preparation for use in compatible PCR workflows according to the applicable assay protocol. Its spectral profile is similar to that of SYBR® Green I and FAM, allowing detection on compatible fluorescence instruments equipped with the appropriate optical channels.
EvaGreen® can also support DNA melt curve analysis, high-resolution melting, digital PCR, and LAMP workflows. Assay performance depends on the reaction composition, dye concentration, instrument configuration, and experimental conditions.
Key Features and Benefits
- Fluorescence-based DNA detection: Produces a fluorescent signal upon binding to double-stranded DNA, supporting monitoring of DNA amplification.
- Low PCR inhibition: Designed to reduce interference with PCR compared with some conventional DNA-binding dyes under suitable reaction conditions.
- High sensitivity: Supports fluorescence detection of amplified DNA and DNA melting behavior in compatible assay systems.
- Real-time PCR compatibility: Suitable for qPCR workflows using compatible fluorescence detection channels and validated protocols.
- Melt curve analysis: Supports post-amplification analysis of DNA melting profiles.
- Multiple molecular biology applications: Can be used in suitable qPCR, HRM®, digital PCR, and LAMP workflows.
- 20X aqueous formulation: Provides a concentrated liquid preparation for convenient incorporation into compatible reaction mixtures.
- Convenient trial size: Supplied in a 1 mL format for laboratory testing and routine research use.
Applications
- Real-Time PCR (qPCR): Fluorescence-based monitoring of DNA amplification during quantitative PCR experiments.
- DNA Melt Curve Analysis: Analysis of DNA melting profiles following amplification to help assess amplicon characteristics and reaction specificity.
- High-Resolution Melting (HRM®): DNA melting analysis for applications such as sequence variation screening when used with compatible instruments and protocols.
- Digital PCR: Fluorescent detection of double-stranded DNA in compatible digital PCR workflows.
- Loop-Mediated Isothermal Amplification (LAMP): Fluorescence monitoring of DNA amplification in suitable isothermal amplification assays.
- DNA Quantification: Fluorescence-based measurement of double-stranded DNA using an appropriate validated method.
- Gel Electrophoresis: PCR products generated with EvaGreen® may be visualized using suitable gel imaging conditions. Confirm compatibility with the assay protocol and detection system.
Product Specifications
| Product Name | EvaGreen® 20X in Water, 1 mL |
|---|---|
| Gentaur Catalog Number | 0037-#31000-T |
| Manufacturer Catalog Number | 31000-T |
| Product Type | Fluorescent nucleic acid dye |
| Formulation | 20X in water |
| Pack Size | 1 × 1 mL |
| Origin | Synthetic |
| UNSPSC | 41106307 |
| Stability | 12 months |
| Shelf Life | 12 months |
| Storage Conditions | Follow the manufacturer's product documentation and label. |
| Dry Ice Required | No |
| Hazardous Product | No |
| Intended Use | Research and molecular biology applications |
Frequently Asked Questions
EvaGreen® is a fluorescent nucleic acid dye that produces increased fluorescence when bound to double-stranded DNA. It is used in fluorescence-based DNA amplification and detection workflows.
The concentrated aqueous formulation is intended for compatible molecular biology workflows, including qPCR, DNA melt curve analysis, HRM®, digital PCR, and LAMP, depending on assay requirements.
EvaGreen® is an alternative DNA-binding fluorescent dye for suitable applications. Its spectral profile is similar to SYBR® Green I, but assay conditions and performance should be validated before substituting one dye for another.
It can be detected using compatible instruments with appropriate fluorescence channels, including commonly used SYBR® Green or FAM detection settings. Instrument and assay compatibility should be confirmed.
Yes. EvaGreen® is used in DNA melt curve workflows and can support high-resolution melting applications with compatible instruments, reagents, and validated protocols.
This product is supplied as a 20X aqueous formulation in a 1 mL package.
The Gentaur catalog number is 0037-#31000-T, and the manufacturer catalog number is 31000-T.
The listed shelf life is 12 months. Consult the product label and manufacturer documentation for storage requirements and expiry details.